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vectastain universal abc kit  (Vector Laboratories)


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    Vector Laboratories vectastain universal abc kit
    Vectastain Universal Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 685 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AK-5200/VECTASTAIN+ABC-AP+Staining+KIT+(Alkaline+Phosphatase%2C+Universal)/pm42887152-329-6-10
    Average 95 stars, based on 685 article reviews
    vectastain universal abc kit - by Bioz Stars, 2026-10
    95/100 stars

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    Amplification:

    Article Title: Identification of Neurite Outgrowth and Synaptic Plasticity-related Genes Showing Sustained Hypermethylation of Promoter Region in the Hippocampal Dentate Gyrus of Rats Exposed Maternally To Neurotoxicants To Induce Persistent Disruption of Hippocampal Neurogenesis.
    Article Snippet: 1 Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan 2 Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan 3 General Toxicology Department, TriApex Laboratories Co., Ltd, No. 9 Xinglong Road, Jiangbei New Area, Nanjing, Jiangsu Province 211800, China 4 Laboratory of Veterinary Anatomy, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan Abstract We previously identified a set of genes with hypermethylated promoter regions that expressed reduced levels of mRNAs by next-generation sequencing in the hippocampal dentate gyrus (DG) of weaned rats [postnatal day (PND) 21] after maternal exposure to valproic acid, propylthiouracil (PTU), or glycidol, compounds that induce persistent disruption of hippocampal neurogenesis until adulthood at PND 77.. The current study was performed to identify immunohistochemically available epigenetic markers of developmental neurotoxicity, focusing on neurite outgrowth and synaptic plasticity-related genes from those identified previously.. After maternal PTU exposure, hypermethylation of Jph3, Kcnj6, and Scn1b at weaning and their persistent reduced expression until adulthood were confirmed by methylation-sensitive high-resolution melting and real-time reverse transcription PCR, respectively.

    Avidin-Biotin Assay:

    Article Title: Identification of Neurite Outgrowth and Synaptic Plasticity-related Genes Showing Sustained Hypermethylation of Promoter Region in the Hippocampal Dentate Gyrus of Rats Exposed Maternally To Neurotoxicants To Induce Persistent Disruption of Hippocampal Neurogenesis.
    Article Snippet: 1 Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan 2 Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan 3 General Toxicology Department, TriApex Laboratories Co., Ltd, No. 9 Xinglong Road, Jiangbei New Area, Nanjing, Jiangsu Province 211800, China 4 Laboratory of Veterinary Anatomy, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan Abstract We previously identified a set of genes with hypermethylated promoter regions that expressed reduced levels of mRNAs by next-generation sequencing in the hippocampal dentate gyrus (DG) of weaned rats [postnatal day (PND) 21] after maternal exposure to valproic acid, propylthiouracil (PTU), or glycidol, compounds that induce persistent disruption of hippocampal neurogenesis until adulthood at PND 77.. The current study was performed to identify immunohistochemically available epigenetic markers of developmental neurotoxicity, focusing on neurite outgrowth and synaptic plasticity-related genes from those identified previously.. After maternal PTU exposure, hypermethylation of Jph3, Kcnj6, and Scn1b at weaning and their persistent reduced expression until adulthood were confirmed by methylation-sensitive high-resolution melting and real-time reverse transcription PCR, respectively.

    Incubation:

    Article Title: Inhibition of de novo ceramide synthesis mitigates alpha-synuclein pathology in a Parkinson's disease mouse model.
    Article Snippet: After washing, sections were blocked with AR TI CL E IN P RE SS 2% normal serum in PBS-T for 2 hours at room temperature, followed by overnight incubation at 4 °C with primary antibody against tyrosine hydroxylase (TH; Novus, OPA1-04050, 1:1000). .. The next day, sections were washed in PBS-T and incubated with a biotinylated anti-rabbit secondary antibody included in the Alkaline Phosphatase Standard Kit (Vector Laboratories, AK-5200) for 30 minutes, followed by alkaline phosphatase reagent (Vector Laboratories, AK-5200) for an additional 30 minutes. .. Signal was developed using Vector® Red Substrate Kit (Vector Laboratories, SK-5100).

    Article Title: Deficiency of Tissue Nonspecific Alkaline Phosphatase Dysregulates Microglial Morphology and Function in a Mouse Model of Infantile Hypophosphatasia
    Article Snippet: Sections were subsequently washed and incubated with biotinylated goat anti‐rabbit IgG antibody (H + L) (RRID: AB_2313606; 1:250; catalog number: BA‐1000‐1.5; Vector Laboratories, CA, USA) for 4 h at room temperature. .. After washing, sections were incubated with VECTASTAIN ABC‐AP Kit (RRID: AB_2336801; catalog number: AK‐5200; Vector Laboratories, CA, USA) for 1.5 h at room temperature and developed using DAB Substrate Kit (3,3′‐diaminobenzidine) (RRID: AB_2336382; catalog number: SK‐4100; Vector Laboratories, CA, USA) following the manufacturer's instructions. .. Slides were dried and cover‐slipped with mounting media (catalog number: F6057; MilliporeSigma, MA, USA).

    Article Title: Inhibition of de novo ceramide synthesis mitigates alpha-synuclein pathology in a Parkinson’s disease mouse model
    Article Snippet: After washing, sections were blocked with 2% normal serum in PBS-T for 2 h at room temperature, followed by overnight incubation at 4 °C with primary antibody against tyrosine hydroxylase (TH; Novus, OPA1-04050, 1:1000). .. The next day, sections were washed in PBS-T and incubated with a biotinylated anti-rabbit secondary antibody included in the Alkaline Phosphatase Standard Kit (Vector Laboratories, AK-5200) for 30 min, followed by alkaline phosphatase reagent (Vector Laboratories, AK-5200) for an additional 30 min. Signal was developed using Vector® Red Substrate Kit (Vector Laboratories, SK-5100). .. Sections were mounted, air-dried, and cover-slipped using Aqueous Mounting Medium (Dako, S3025).

    Staining:

    Article Title: Bidirectional Modulation of Beam Traversal Performance by Acetylcholine in the Cerebellar Nuclei
    Article Snippet: .. Post-recording, slices were preserved in 4% PFA and stained for biocytin using the ABC reagent (Vector Labs, VECTASTAIN ABC AP Kits), followed by DAB staining with Nickel (Vector Labs, DAB Substrate Kit, Peroxidase HRP). ..

    Article Title: Development and preclinical characterization of an MMAE-conjugated anti-EpCAM antibody-drug conjugate for colorectal cancer.
    Article Snippet: .. Immunohistochemical staining was performed using the VECTASTAIN Universal ABC Kit (Vector Laboratories) according to the manufacturer’s instructions. .. Tissue sections were blocked for 1 h and subsequently incubated with anti-EpCAM #126 (laboratory-produced) or adecatumumab (MedChemExpress) at a final concentration of 10 μg/ mL for 4 h at 25 ◦C.

    Immunohistochemical staining:

    Article Title: Development and preclinical characterization of an MMAE-conjugated anti-EpCAM antibody-drug conjugate for colorectal cancer.
    Article Snippet: .. Immunohistochemical staining was performed using the VECTASTAIN Universal ABC Kit (Vector Laboratories) according to the manufacturer’s instructions. .. Tissue sections were blocked for 1 h and subsequently incubated with anti-EpCAM #126 (laboratory-produced) or adecatumumab (MedChemExpress) at a final concentration of 10 μg/ mL for 4 h at 25 ◦C.



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